An inter-platform repeatability study investigating real-time amplification of plasmid DNA
نویسندگان
چکیده
منابع مشابه
An inter-platform repeatability study investigating real-time amplification of plasmid DNA
BACKGROUND The wide variety of real-time amplification platforms currently available has determined that standardisation of DNA measurements is a fundamental aspect involved in the comparability of results. Statistical analysis of the data arising from three different real-time platforms was conducted in order to assess inter-platform repeatability. On three consecutive days two PCR reaction mi...
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Real-time quantitative PCR (QPCR) has been proven to be a powerful tool for quantifying specific target DNA sequences. Compared to relative quantification, absolute quantification has the advantage of determining the absolute copy number of a given target, such as pathogen or plasmid DNA in vivo. However, matrix or impurities remaining in a DNA sample after various sample treatment procedures m...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2005
ISSN: 1472-6750
DOI: 10.1186/1472-6750-5-15